EasyLife X

EasyLife™ X Hardware

 
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EasyLife XWe just can't emphasize enough how easy the EasyLife™ is to perform fluorescence lifetime experiments. There is a power button to turn on the instrument, and there is dial to adjust the stirring rate of the optional micro-stirrer for the cuvette. Everything else is controlled through the software. It is just that easy!

 

 

 

Pulsed LED Light Sources

EasyLife LEDNothing could be easier. OBB engineers have developed a broad range of simple, inexpensive, nanosecond pulsed light emitting diodes (LED's) to be used as excitation sources with the EasyLife™ systems. These small but robust light sources are available in a broad range of wavelengths, from UV to NIR. They have excellent pulse to pulse, and long term, stability in their intensity as well as their temporal pulse profiles as is evidenced by the high quality of data the system produces. Switching from one LED to another requires no alignment, and takes only a few seconds. You could not possibly work with any simpler, maintenance-free, light source for fluorescence lifetime research.

 

EasyLife LED Spectra

 

EasyLife LED Relative Intensities

 

 

Pulsed LED Advantages

The EasyLife™ pulsed LEDs have certain distinct advantages over more conventional light sources, such as nanosecond flash lamps and lasers.

These advantages are:

  • Inexpensive
  • Durable
  • Require no maintenance
  • Very stable pulse sources
  • Fast
  • Wavelengths from UV to NIR (currently 280–670 nm)
  • Small size
  • Easily interchangeable (snap in—snap out)

Impressive Pulse Stability

EasyLife Stability Test EasyLife Stability Test

6 Hours Repeat Acquisition

Repeat After 4 Days

Click on images to enlarge.

Available LEDs

What you need to do is to select a source or sources. The following list shows you the current choices. To exchange sources is real easy, just snap off and then snap on and it is done—no alignment required.

Below 280 nm we are limited by the current state of the technology for LED’s. Do not despair the future should offer even more choices.

LED Sources:

Central Wavelength

EL 265

excitation LED: 266 +\- 10 nm

EL 278

excitation LED: 280 +\- 10 nm

EL 295

excitation LED: 297 +\- 10 nm

EL 310

excitation LED: 310 +\- 10 nm

EL 340

excitation LED: 340 +\- 10 nm

EL 366

excitation LED: 368 +\- 10 nm

EL 375

excitation LED: 375 +\- 10 nm

EL 405

excitation LED: 403 +\- 10 nm

EL 410

excitation LED: 407 +\- 10 nm

EL 435

excitation LED: 432 +\- 10 nm

EL 445

excitation LED: 444 +\- 10 nm

EL 450

excitation LED: 456 +\- 10 nm

EL 490

excitation LED: 486 +\- 10 nm

EL 505

excitation LED: 510 +\- 10 nm

EL 525

excitation LED: 518 +\- 10 nm

EL 570

excitation LED: 572 +\- 10 nm

LD 630

excitation laser diode: 633 +\- 10 nm

LD 650

excitation laser diode: 649 +\- 10 nm

LD 670

excitation laser diode: 667 +\- 10 nm

Other LED's and laser diodes are available on request.

 

Sample Compartment

The EasyLife™ sample compartment can be configured with a variety of options including the following.

  • micro-stirrer
  • bandpass and longpass filters
  • neutral density filters
  • cold finger dewar

Unique Patented Detection

The EasyLife™ X is a time-domain fluorescence lifetime system. It collects a decay curve, allowing the user to fit the curve with comprehensive analysis software that provides many statistical tools to assess goodness of fit. The particular time-domain detection technique used in the EasyLife™ X is called the Stroboscopic technique and it is patented and unique. The Stroboscopic technique was initially developed  to be a simpler, less expensive alternative to Time-Correlated Single-Photon-Counting (TCSPC). Consequently the EasyLife™ X is the world’s least expensive fluorescence lifetime system.

Lifetime-Based Reaction Kinetics Acquisition Protocol

In addition to acquire fluorescence decays, the EasyLife™ X has the ability to measures Lifetime-Based Reaction Kinetics. If you are familiar with using a fluorometer for time-based kinetics you are familiar with collecting the steady state intensity as a function of time to monitor kinetics and reactions. The EasyLife™ X can acquire data in a similar way except that instead of the intensity changing with time the EasyLife™ X acquires, and plots in real time, the measured lifetime as a function of time. This acquisition protocol lets you measure changes in lifetimes rather than intensity. The time duration of this measurement can be from a few seconds to many hours. We believe this capability, will become an essential tool for a wide variety of emerging applications.

*If your kinetics occur on a faster timeframe then consider our new DeltaPro system that can measure lifetimes in as short a time as 1 millisecond.

Unique Logarithmic/Arithmetic Time Scale Decay Acquisition

The EasyLife™ X software takes full advantage of the patented stroboscopic detection hardware, to provide another unique acquisition protocol. Collecting data in a logarithmic, or arithmetic, time scale can be extremely useful for samples that have both very short and very long fluorescence lifetimes. For the short lived components you need to collect a lot of data points in order to get a good fit for those lifetimes, but for the very long lifetimes you don’t want to collect data at the same time resolution because it takes much longer and provides no benefit in fitting the decay curve.

Systems that use other techniques like TCSPC collect data in a linear time scale, so for this sample they necessarily must collect many more data points across the full acquisition time window in order to have enough data points to resolve the short lifetime components. In this way the Stroboscopic  technique employed with the EasyLife™ X can easily collect excellent data for complex decay phenomena.

EasyLife™ X System

The entire EasyLife™ X takes up just one cubic foot of lab space!

With our proprietary LED light sources combined with our patented stroboscopic detection system, you are able to reap the benefits of high sensitivity, excellent temporal resolution and the unique ability to acquire lifetime kinetics or to utilize both linear and non-linear (arithmetic and logarithmic) acquisition time base. The result is a powerful and versatile lifetime instrument, capable of handling complex multi-exponential decays with an underlying broad range of lifetimes.

Did we mention that the EasyLife™ X is the easiest system available to do fluorescence lifetime experiments?

For more information on the EasyLife™ X visit the EasyLife™ X Software page.

 
OBB has a policy of continuous product development and reserves the right to amend part numbers, descriptions and specifications without prior notice.
 
 
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Edison New Jersey
08820-3097

Tel: 732-494-8660
Fax: 732-623-8129
E-mail:info.sci@horiba.com