EasyLife X

EasyLife™ X Software

 
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EasyLife™ software is so easy to use that literally anyone can use the system. A critical requirement to making a fluorescence lifetime system easy to use is the software, and the EasyLife™ software does the job. This is how simple the software is to use.

Just Two Steps

1. Select your Fluorescence Decay acquisition. Hit the Acquire button and within seconds or minutes depending on your settings you will have collected a decay curve. Repeat the acquisition for a scatter sample to acquire an instrument response function if necessary.

Aquire Fluorescence Decay

2. Analyze the decay: From the Math pull down list, select the decay model you wish to employ to fit the decay curve. Stretch the analysis window across the decay curve to determine the range for the fit. Under Start Parameters, check the number of components you want to fit. Then hit the Start Fit button. The EasyLife™ software automatically displays the resultant fitted decay curve, the measured lifetimes and a number of goodness of fit parameters such as CH1 Squared, the plot of the residuals and more. It just doesn't get any easier than that.

Analyze 1-4 Exponential

EasyLife™ software is so powerful you will be shocked at how much you get with your system

Don't let the price of the EasyLife™ fool you. This system comes with a totally complete set of acquisition and analysis tools for time resolved fluorescence.

Lifetime-Based Reaction Kinetics Acquisition Protocol

In addition to acquire fluorescence decays, the EasyLife™ X has the ability to measures Lifetime-Based Reaction Kinetics. If you are familiar with using a fluorometer for time-based kinetics you are familiar with collecting the steady state intensity as a function of time to monitor kinetics and reactions. The EasyLife™ X can acquire data in a similar way except that instead of the intensity changing with time the EasyLife™ X acquires, and plots in real time, the measured lifetime as a function of time. This acquisition protocol lets you measure changes in lifetimes rather than intensity. The time duration of this measurement can be from a few seconds to many hours. We believe this capability, will become an essential tool for a wide variety of emerging applications.

*If your kinetics occur on a faster timeframe then consider our new DeltaPro system that can measure lifetimes in as short a time as 1 millisecond.

Unique Logarithmic/Arithmetic Time Scale Decay Acquisition

The EasyLife™ X software takes full advantage of the patented stroboscopic detection hardware, to provide another unique acquisition protocol. Collecting data in a logarithmic, or arithmetic, time scale can be extremely useful for samples that have both very short and very long fluorescence lifetimes. For the short lived components you need to collect a lot of data points in order to get a good fit for those lifetimes, but for the very long lifetimes you don’t want to collect data at the same time resolution because it takes much longer and provides no benefit in fitting the decay curve.

World Class Decay Analysis Software

EasyLife™ X comes with a powerful lifetime analysis package comprising 8 different fitting programs and a FRET calculator, covering virtually all possible application scenarios. The software even includes distribution analysis for complex multi-component decay analysis. All of the fitting programs employ deconvolution as a standard option. Deconvolution removes the distortion imposed on a decay curve by a finite temporal width of the excitation pulse. It makes it possible to determine lifetimes that are 10x shorter than the excitation pulse. In order to use the deconvolution option the user must acquire an instrument response function (IRF) in addition to the fluorescence decay. The IRF can be measured by replacing the fluorescent sample with a scatterer.

Distribution analysis of multi-exponential decay

MEM decay with EasyLife™ X

The fluorescence decay of CdSe quantum dots measured with the EasyLife™ X indicates a highly heterogeneous nature of the sample. Given that this sample had multi-exponential decays with an underlying broad range of lifetimes, the unique arithmetic progression timescale acquisition was used with this sample to greatly improve the acquisition time and analysis. This result was validated by the ESM lifetime distribution analysis tool which not only confirmed the lifetime values from the discrete 4-exponential analysis, but provides valuable information about the distribution of those discrete lifetimes

 

The following analysis programs are included with every EasyLife™ X 

Click to Expand

1-to-4 exponentials

This program is suitable for the analysis of fluorescence decays consisting of up to 4 exponentials and associated pre-exponential factors. This is the most commonly used program in lifetime analysis.

Multi 1-to-4 exponentials

The multiple file 1-to-4 exponential lifetime method allows the analysis of multiple scatterer/sample pairs as a batch operation. Each pair will be separately analyzed over the same range with the same number of exponentials and the same options. This type of analysis is useful when a series of decay curves has been collected as a function of some parameter (i.e. concentration of added reagent). Trends in the values of the lifetime parameters may then be recognized rather easily.

Global 1-to-4 exponentials

This program analyzes decays with up to 4 lifetimes for a number of data files simultaneously. The global analysis assumes that the lifetimes are the same among the data files but that the associated pre-exponentials are free to vary. For example, the global can be useful for analyzing various mixtures of up to 4 fluorophores.

Anisotropy decay

This program is used to calculate rotational correlation times plus a residual anisotropy term. Optional polarizers are required. The program first allows the user to calculate the fluorescence lifetime(s) from the parallel and perpendicularly polarized emission intensities. The user can then calculate the rotational correlation time(s).

Micelle kinetics

This program uses a “stretched exponential” fitting function (Infelta-Graetzel eqn.), which describes the quenching in micelles when added quencher molecules are Poisson distributed among the micelles. The analysis allows the determination of micellar aggregation number and the quenching rate constant.

MEM lifetime distribution

The Maximum Entropy Method (MEM) is designed to recover lifetime distributions without any a priori assumptions about their shapes. This method uses a series of exponentials (up to 200 terms) as a probe function with fixed, logarithmically spaced lifetimes and variable pre-exponentials. This allows analyzing fluorescence decays with underlying lifetimes spanning several orders of magnitude. In many situations the MEM is capable of differentiating between continuous distributions and discrete, multi-exponentials decays. The algorithm minimizes chi-square while maximizing the entropy function at each iteration step. Ideal for complex decays, such as labeled proteins and membranes, probes adsorbed on surfaces, probes with conformational flexibility, polymers etc.

ESM lifetime distribution

Similar to MEM, but without entropy maximization, the Exponential Series Method (ESM) is designed to recover lifetime distributions without any a priori assumptions about their shapes. This method uses a series of exponentials (up to 200 terms) as a probe function with fixed, logarithmically spaced lifetimes and variable pre-exponentials. This allows analyzing fluorescence decays with underlying lifetimes spanning several orders of magnitude. In many situations the ESM is capable of differentiating between continuous distributions and discrete, multi-exponentials decays. Ideal for complex decays, such as labeled proteins and membranes, probes adsorbed on surfaces, probes with conformational flexibility, polymers etc.

Non-exponential

This program allows for the analysis of data by a fitting function consisting of two exponentials multiplied together and each with variable exponents of time. The exponents can be either varied or fixed which provides a powerful general function for models such as Förster energy transfer, time-dependent quenching and molecular interaction in restricted geometries (e.g. molecules on surfaces, zeolites etc.).

FRET calculator

Forster Resonance Energy Transfer (FRET), sometimes referred to as Fluorescence Resonance Energy Transfer is an extremely powerful technique for the study of molecular interactions. Fluorescence lifetimes have become the technique of choice for doing FRET experiments because investigators have come to realize that FRET experiments based on steady state fluorescence measurements can sometimes result in erroneous results due to spectral shifts resulting in something other than actual FRET. The fluorescence lifetime technique lets you know if what you are looking at is really FRET. The EasyLife™ FRET Calculator calculates basic FRET parameters, such as FRET efficiency, FRET rate constant, D-A distance, and Forster radius Ro (requires spectral data from a fluorometer).

Trace Math

The commands in the Trace Math menu allow specific mathematical functions and operations to be carried out on individual traces or selected regions of a trace. There are 16 functions including antilog, average, distribution average, combine, xy combine, multiple derivatives, integration, linear fit, linear scaling, logarithm, normalization, reciprocal, smoothing, truncation, baseline suppression and trace merging. There is also a peak finder provided. All these functions can greatly facilitate data processing and presentation.

 

EasyLife™ QuickStart DVD

The EasyLife™ software that comes with the system can be loaded onto your Windows XP® laptop or computer. It loads easily on the computer and self calibrates with the instrument. Along with the QuickStart DVD you will be able to get started acquiring data right away. However, the compatibility of the EasyLife™ software with customer supplied computers is not the responsibility of OBB Corp. If you are concerned about providing your own compatible laptop or computer, we can include a laptop (with EasyLife™ software pre-loaded) on your EasyLife™ quotation so that it comes with your system. If you do decide to provide your on computer, below are the minimum specification requirements.

Computer Requirements

  • Microsoft Windows XP® with SP2
  • Intel Pentium® III class processor, 500 MHz or higher
  • USB port (USB 2 recommended)
  • Video display 800 x 600, 256 colors or higher
  • Minimum of 256 MB of RAM (512 MB or higher recommended)
  • Minimum of 125 MB free hard drive space
  • Microsoft® compatible mouse

For more information on the EasyLife™ X visit the EasyLife™ X Specifications page.

 
OBB has a policy of continuous product development and reserves the right to amend part numbers, descriptions and specifications without prior notice.
 
 
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