EasyLife L

EasyLife™ L Applications

 
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The EasyLife™ L can be used for a wide variety of luminescence applications.

  • DNA-Lanthanide assays for multi-drug resistance of TB strains etc.
  • DNA-Lanthanide Binding Kinetics
  • LRET based kinase activity assays
  • Conformational changes in ligand-binding glutamate receptor
  • Sigma factor binding to RNA polymerase
  • K+ channel voltage sensor movement in cell membranes
  • Detection of salicylic acid in blood by sensitized Tb luminescence
  • Assays for determining antibiotics (norfloxacin, garenoxacin, grepafloxacin) in urine and serum
  • LRET based protease assay to detect b-amyloid cleaving enzyme (BACE) activity,
  • Kinase assays using terbium -fluorescein and terbium-GFP fusions of native physiologically relevant substrates
  • δ 70 factor binding to RNA polymerase
  • Inhibitors that inhibits s70 binding to RNA polymerase
  • RNAP holoenzyme binding to DNA
  • The apo state structure of the ligand binding domain and the conformational changes induced by agonist binding to ionotropic glutamate Kainate receptor
  • Conformational changes in glutamate transporters
  • Conformational change of AMPA receptors upon glutamate binding
  • Myosin conformation and conformational change within myosin during muscle contraction
  • Connexin 43 gap-junctional hemichannels subunit composition change after Phosphorylation by PKC
  • Topology study of prokaryotic voltage-gated ion channels NaChBac and KVAP,in the lipid bilayer
  • n situ measurement of atomic scale movement in the voltage-sensing region of a potassium channel Shaker potassium channel subunits in response to electric potential
  • Distribution of DNA bend angles in catabolite activator protein (CAP)-DNA complex in solution
  • 17β-estradiol (E2) binding to E2 specific antibody
  • Structure and conformation of the photoreceptor rhodopsin (Rh) in the membrane as a dimer or multimer

 

Quattro Google Scholar searchAlthough the EasyLife™ L is a new system there are a growing number of publications citing the use of the EasyLife™ L.

To view an updated list of these citations on Google Scholar, click here.

 

DNA-Lanthanide assays for multi-drug resistance of TB strains etc

The time resolved technique is vastly superior to a steady state luminescence assay as it is insensitive to artifacts that can affect intensity, such as light scattering, concentration fluctuations and precipitation. Additionally the lifetime technique directly measures the fraction of bound versus unbound probe, whereas a steady state assay has no way to do this.

TB+DNA Decay

DNA-lanthanide assays are used in a number of diagnostic tests, for example DNA-Tb3+ in testing for antibiotic resistance of tuberculosis strains. Terbium luminescence is enhanced upon binding to DNA, which results in 3 fold increase of Tb3+ lifetime.

 

DNA-Lanthanide Binding Kinetics

The Lifetime Kinetics acquisition protocol is unique to the EasyLife™ L and allows for the study of binding kinetics based on the lifetime of the probe. Lifetime Kinetics is totally independent of intensity fluctuations caused by artifacts such as light scattering, concentration fluctuations and precipitation.

TB+DNA Decay

The binding kinetics of Tb3+ to DNA can be followed free of intensity artifacts by using the unique Lifetime Kinetics acquisition mode. Here the EasyLifeT L performs continuous rapid measurements of the average terbium lifetime in real time.

 
OBB has a policy of continuous product development and reserves the right to amend part numbers, descriptions and specifications without prior notice.
 
 
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